ABSTRACT
Lutzomyia longipalpis es el principal v ector de una importante enfermedad desatendida en América. La diversidad genética de este vector se estimó en la población colectada en dos áreas geográficas separadas por hasta 37 km. Analizamos la secuencia CB3PDR / N1NPDR de 22 individuos obte niendo un parámetro de: h = 0.43 y π = 0.0017 (Bona), h = 0.89, π = 0.004 (El Limón) con una dife renciación genética de kst = 0.03; p> 0.05 entre ellos. Ocho haplotipos fueron detectados, de los cuales fue compartido. Se detectó una diferenciación significativa entre las poblaciones Panamá Colombia (kst = 0.98), PanamáCosta Rica (kst = 0.98) y PanamáBrasil (kst = 0.72) bajo el modelo de aislamiento. Las inferencias genéticas de esta población pueden complementar la información de la capacidad de dispersión y brindar pistas importantes para comprender la ecología de Lutzomyia longipalpisen Panamá.
Lutzomyia longipalpis is the main vector of an important neglected disease in America. The genetic div ers ity of this vector was estimated in the population collected in two geographical areas separated by up to 37 km. We analyzed the sequence CB3PDR / N1NPDR of 22 individuals obtaining a parameter of: h = 0.43 and π = 0.0017 (Bona), h = 0.89, π = 0.004 (The Lemon) with a genetic differentiation of kst = 0.03; p> 0.05 between them. Eight haplotypes were detected, of which it was shared. A significant differentiation was detected between the PanamaColombia (ks t = 0.98), PanamaCosta Rica (kst = 0.98) and PanamaBrazil (kst = 0.72) populations under the isolation model. The genetic inferences of this population can complement the dispersion information and provide important clues to understand the ecology of Lutzomyia longipalpis in Panama.
Subject(s)
Psychodidae/pathogenicity , Leishmaniasis/epidemiology , Genes, Mitochondrial/genetics , Electron Transport Complex IV/geneticsABSTRACT
In Panama, species of the genus Lutzomyia are vectors of American cutaneous leishmaniasis (ACL). There is no recent ecological information that may be used to develop tools for the control of this disease. Thus, the goal of this study was to determine the composition, distribution and diversity of Lutzomyia species that serve as vectors of ACL. Sandfly sampling was conducted in forests, fragmented forests and rural environments, in locations with records of ACL. Lutzomyia gomezi, Lutzomyia panamensis and Lutzomyia trapidoi were the most widely distributed and prevalent species. Analysis of each sampling point showed that the species abundance and diversity were greatest at points located in the fragmented forest landscape. However, when the samples were grouped according to the landscape characteristics of the locations, there was a greater diversity of species in the rural environment locations. The Kruskal Wallis analysis of species abundance found that Lu. gomezi and Lu. trapidoi were associated with fragmented environments, while Lu. panamensis, Lutzomyia olmeca bicolor and Lutzomyia ylephiletor were associated with forested environments. Therefore, we suggest that human activity influences the distribution, composition and diversity of the vector species responsible for leishmaniasis in Panama.
Subject(s)
Animals , Female , Male , Genetic Variation , Insect Vectors/classification , Psychodidae/classification , Leishmaniasis, Cutaneous/transmission , Panama , Population Density , Population Dynamics , SeasonsABSTRACT
Lutzomyia longipalpis is the primary vector of the parasite responsible for visceral leishmaniasis in the Americas. In the present study, Lu. longipalpis was found in a domiciliary area in Limón, a district in Capira, a region in which cutaneous leishmaniasis is endemic in Panama. Previously, this species has been found in a humid forest in this same region. Finding Lu. longipalpis in domiciliary areas indicates that this species may be adapting to new habitats and that it may play a role in the transmission of leishmaniasis in Panama.
Subject(s)
Animals , Female , Insect Vectors/classification , Leishmaniasis, Cutaneous/transmission , Psychodidae/classification , Endemic Diseases , Leishmaniasis, Cutaneous/epidemiology , Panama/epidemiology , SeasonsABSTRACT
Informa que el objetivo es determinar la situación actual de la epidemiología de las arbovirosis zoonóticas tales como Encefaliomielitis de San Louis, Encefalomielitis Equina del Este, Encefalomielitis Equina Venezolana y Virus del Nilo Occidental en Panamá, detectando los virus en sus reservorios y transmisores; utilizando como harramientas las técnicas serológicas, virológicas y moleculares.